A CENTRIFUGAL MICROFLUIDIC METHOD FOR ENRICHMENT AND ENZYMATIC EXTRACTION OF SARS-COV-2 RNA FROM CLINICAL SAMPLES

The requirement for diversification of methods for sample preparation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is increasingly important to circumvent analysis bottlenecks and continue widespread surveillance testing. We report a centrifugally-driven microfluidic platform for automated affinity nanoparticle enrichment and enzymatic RNA extraction of SARS-CoV-2. The microfluidic disc (μCD) and accompanying mechatronic system(s) are capable of sample preparation from up to six patient samples simultaneously and under 20 minutes. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.